Journal: Cancers

Article Title: Murlentamab, a Low Fucosylated Anti-Müllerian Hormone Type II Receptor (AMHRII) Antibody, Exhibits Anti-Tumor Activity through Tumor-Associated Macrophage Reprogrammation and T Cell Activation

doi: 10.3390/cancers13081845

Figure Lengend Snippet: Flow cytometry antibodies used.

Article Snippet: CD3-VB , Miltenyi , 130-133-133 , BW264/56 , .

Techniques: Flow Cytometry, In Vivo, In Vitro

Journal: Cancers

Article Title: Murlentamab, a Low Fucosylated Anti-Müllerian Hormone Type II Receptor (AMHRII) Antibody, Exhibits Anti-Tumor Activity through Tumor-Associated Macrophage Reprogrammation and T Cell Activation

doi: 10.3390/cancers13081845

Figure Lengend Snippet: Murlentamab opsonization of SKOV3-R2 + activates an effective anti-tumor T cell immune response. SKOV3-R2 + ovarian tumor cells were labeled with different 3C23K antibodies (3C23K-FcKO control, 3C23K-CHO normally fucosylated or murlentamab the low fucosylated form) and cultured in the presence of human monocyte-derived macrophages from healthy donors unstimulated (M0) or stimulated with M-CSF and IL-10 (TAMs). After 3 days of co-culture, activated T cells coming from the same healthy donor were added in the culture well for 4 more days. ( A ) The CD4 + Th1/Th2 polarization profile, ( B ) the proportion of CD3 + CD4 + CD25 + regulatory T cells and ( C ) the activation of T CD8 + cells were determined by flow cytometry after four days of co-culture. Data shown (boxplots) are the results from two different experiments (performed with two different healthy donors). * p < 0.05; ** p < 0.01; *** p < 0.001. p values were determined using one-way ANOVA analysis followed by Tukey’s multiple comparisons test.

Article Snippet: CD3-VB , Miltenyi , 130-133-133 , BW264/56 , .

Techniques: Labeling, Cell Culture, Derivative Assay, Co-Culture Assay, Activation Assay, Flow Cytometry

Journal: OncoTargets and therapy

Article Title: Integrated RNA Sequencing and Single-Cell Mass Cytometry Reveal a Novel Role of LncRNA HOXA-AS2 in Tumorigenesis and Stemness of Hepatocellular Carcinoma

doi: 10.2147/OTT.S272717

Figure Lengend Snippet: Purified Antibodies About the Stem-Like Cells Centric Panel

Article Snippet: Met , 167Er , D1C2 , Fluidigm , 3167017A.

Techniques: Purification

Journal: Frontiers in Oncology

Article Title: Intrinsic Differences in Spatiotemporal Organization and Stromal Cell Interactions Between Isogenic Lung Cancer Cells of Epithelial and Mesenchymal Phenotypes Revealed by High-Dimensional Single-Cell Analysis of Heterotypic 3D Spheroid Models

doi: 10.3389/fonc.2022.818437

Figure Lengend Snippet: List of IMC antibodies.

Article Snippet: 167 Er , MET §§ , D1C2 , 3167020D , Fluidigm , 1:50.

Techniques:

Journal: Mediators of Inflammation

Article Title: The Role of EGFR/PI3K/Akt/cyclinD1 Signaling Pathway in Acquired Middle Ear Cholesteatoma

doi: 10.1155/2013/651207

Figure Lengend Snippet: The cultured cells grew into monolayer and showed a polygonal “cobblestone-like” appearance (a). The cultured cells showed a positive reaction to cytokeratins by immunofluorescence, and the signal was detected in cytoplasm (b).

Article Snippet: The cells were then incubated with primary mouse antibody against cytokeratins (1 : 150 dilution; Boster, China) at 37°C for 90 min. After incubation, the cells were washed in PBS and incubated with TRITC-labeled goat-anti-mouse secondary antibody (1 : 50 dilution; Boster, China) at 37°C for 60 min. Coverslips were finally mounted for observation.

Techniques: Cell Culture, Immunofluorescence